The shell, protoconch, radula, head, penis, and female reproductive organs are described and illustrated for Greek Pseudamnicola macrostoma (Kuster) from two localities in Attica, P. negropontina (Clessin) from Evvia Island, and Adrioinsulana conovula (Frauenfeld) from Pag Island in Croatia. The two populations of P. macrostoma were sampled in 1985, and both were later destroyed. P. negropontina was sampled again in 2003, and the material was used for extraction of DNA. Four sequences of mitochondrial CO1 gene were obtained. The parsimony-based molecular phylogeny (with eight sequences from the GenBank, including P. lucensis (Issel) and Adrioinsulana conovula) showed that the Greek P. negropontina actually belongs to the genus Pseudamnicola, and that the molecular differences between P. negropontina and Italian P. lucensis are twice greater than between the latter and A. conovula, thus distinguishing the genus Adrioinsulana is not justified: all the species belong to Pseudamnicola.